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Equipment Info
Super Resolution Confocal Microscope Facility
Equipment Code : 2745725
Make : Nikon and Visitech
Model : Nikon Confocal AXR, Super Resolution, VT i SIM, Nikon Super Resolution N STORM
Institution : Indian Institute of Technology (IIT) Bombay
Department : Centre for Sophisticated Instruments and Facilities
Funding Agency Details :
Pooling of Equipment slot : No
Last AMC Done :
Reference Website : https://instruments.iitb.ac.in/super-resolution-confocal-microscope-facility
Description : Super-resolution microscopy is a relatively new method compared to confocal microscopy, and ongoing developments aim to improve its standards. While confocal microscopy has been praised for its high contrast, recent methods show that super-resolution microscopy, specifically via theĀ instant-structured illumination microscopy (i-SIM) technique, is capable of capturing reflectance imaging with more than double the resolution of confocal microscopy. This increased resolving capability has been applied to imaging nanoparticle uptake and localization inside cells. These developments contribute to super-resolution microscopy becoming a validated alternative to confocal microscopy for imaging subcellular activity. Whilst maintaining the necessary temporal resolution and at a low photo bleaching level, VT-iSIM offers a spatial resolution of 100nm and axial resolution of 300nm.Super-resolution microscopy allows us to see the smallest structures in living cells that are not visible with standard
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SRM (min slot 2 hours)
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Note:
i) Academic (External) means researchers belongs to the other institution.
ii) Academic (Internal but different department/lab) means researchers belongs to other department/lab or other investigators.
iii) Academic (Internal) means researchers belongs to the same institution.
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