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Equipment Info
Orbitrap High Resolution Liquid Chromatograph Mass Spectrometer (HRLCMS)
Equipment Code : 2736837
Make : Thermo Fisher Scientific Pte. Ltd.
Model : Q-Exactive Plus Biopharma-High Resolution Orbitrap
Institution : Sophisticated Analytical Instrument Facilities (SAIF) IIT Bombay
Department : SAIF
Funding Agency Details : DST, Govt. of India
Pooling of Equipment slot : No
Last AMC Done :
Reference Website : www.saif.iitb.ac.in
Description : The Orbitrap is an ion trap mass analyzer that consists of two outer electrodes and a central electrode, which enable it to act as both an analyzer and detector. Ions entering the Orbitrap are captured through "electrodynamic squeezing," after which they oscillate around the central electrode and in between the two outer electrodes. Different ions oscillate at different frequencies, resulting in their separation. By measuring the oscillation frequencies induced by ions on the outer electrodes, the mass spectra of the ions are acquired using image current detection. Liquid chromatography / Mass Spectroscopy (LC / MS) is a technique which combines high performance liquid chromatography HPLC, a powerful analytical separation technique with mass spectroscopy,a powerful analysis & detection technique. There are two common atmospheric pressure ionization (API) LC/MS process: Electrospray Ionization (ESI) & Atmospheric Pressure Chemical Ionization (APCI). Both are soft ionization technique.
| Samples | External Academic User | Internal Academic User | International User | Industry User | Start-ups | National Research Lab User | Self (Project Investigator) |
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Only HRMS
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HRMS + compound formula & library search
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LCMS with library (Plant extract or Impurity Profiling or Metabolite Identification or Degradation studies or Environmental samples)
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Protein Identification (for 1-hour)
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Protein Identification (for 2-hours)
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Sample Preparation (In-gel or In-solution Trypsin Digestion with Desalting)
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Only desalting of digested protein samples
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Lipidomics
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Intact Mass of Proteins Peptides and Oligonucleotides
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Note:
i) Academic (External) means researchers belongs to the other institution.
ii) Academic (Internal but different department/lab) means researchers belongs to other department/lab or other investigators.
iii) Academic (Internal) means researchers belongs to the same institution.
| Sample Name | Sample Type | Sample UOM | Sample Availability | Unit Cost | Sample Quantity | Sample Description |
|---|---|---|---|---|---|---|
| No Data Available | ||||||
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